Research Article

Tumor Suppressor miR-613 Alleviates Non-Small Cell Lung Cancer Cell via Repressing M2 Macrophage Polarization

Figure 6

miR-613 influences tumor growth by reducing the M2 macrophage polarization in vivo. Macrophages with different treatments (as in Figure 4) were co-cultured with H1650 cells. H1650 cells were grouped: H1650+MNCmimic, H1650+MIL-4+NCmimic and H1650+MIL-4+miR-613mimic. The C57BL/6 mice were subcutaneously injected with the above H1650 cells (5 × 105 cells). (a, b) Analysis of the tumor weight. (c) The proliferation and apoptosis were analyzed by Ki-67 staining and TUNEL staining (scale bar: 100 μm). (d) miR-613 expression in tumors by qRT-PCR. vs. H1650+MNCmimic. , vs. H1650+MIL-4+NCmimic.
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