Silencing UBQLN2 Enhances the Radiosensitivity of Esophageal Squamous Cell Carcinoma (ESCC) via Activating p38 MAPK

Figure 3

Silencing UBQLN2 enhanced the radiosensitivity of esophageal squamous cell carcinoma (ESCC) cells. The radiosensitivity of wild-type (WT) or UBQLN2 knockdown (UBQLN2-KD) Ec109 (a) and KYSE-30 (b) cells was detected by a clone formation assay. Representative pictures of the colony formation assay are given. Western blotting analysis showed that silencing UBQLN2 could significantly inhibit the protein expression of the phosphorylated DNA-dependent protein kinase catalytic subunit (p-DNA-PKcs) in Ec109 (c) and KYSE-30 (d) cells after irradiation (IR). All results are presented as the mean ± SEM from three repeated experiments.  < 0.05 and  < 0.01 of analysis of variance (ANOVA).

(a)

(b)

(c)

(d)