FOXO1-Induced miR-502-3p Suppresses Colorectal Cancer Cell Growth through Targeting CDK6

Figure 3

FOXO1 elevates miR-502-3p level through facilitating MIR502 transcription in CRC cells. (a) Potential FOXO1 binding site on MIR502 promoter analyzed using HumanTFDB database. (b) The protein level of FOXO1 in FHC cells and HT29 cells detected by WB. (c) The level of miR-502-3p in HT29 cells transfected with or without FOXO1 expression vector identified by qRT-PCR. (d) Luciferase activity of HT29 cells co-transfected with reporter gene vectors containing the promoter of MIR502 and FOXO1 expression vector. Vec: expression vector; OE: overexpression. N = 3. , , .

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