FOXO1-Induced miR-502-3p Suppresses Colorectal Cancer Cell Growth through Targeting CDK6

Figure 4

MiR-502-3p targets CDK6 in CRC cells. (a) miR-502-3p binding site on CDK6 mRNA 3′ UTR analyzed using ENCORI database. (b) The protein level of CDK6 in HT29 cells treated with or without miR-502-3p mimic detected by WB. (c) Luciferase activity of HT29 cells co-transfected with reporter gene vectors containing the binding site of miR-502-3p on CDK6 mRNA 3′ UTR and miR-502-3p mimic or inhibitor. N = 3. NC: negative control; WT: wildtype; MUT: mutant. , , .

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