LncRNA SNHG5 Suppresses Cell Migration and Invasion of Human Lung Adenocarcinoma via Regulation of Epithelial-Mesenchymal Transition

Figure 2

In LAD A549 cells, overexpression of SNHG5 decreases the expression of EMT-associated genes. (a) To evaluate the effect of lncRNA SNHG5 on lung A549 cells, transcriptome sequencing analysis (GSE143766) was performed. According to the color bar in the logarithmic scale given above the heatmap, the red and green colors in the heatmap respectively reflect high and low expression of target genes. (b) Volcano plot analysis of the differential gene expression after SNHG5 overexpression. (c) KEGG analysis of downregulated genes in SNHG5-overexpressing A549 cells. (d) The qRT-PCR analyzed the expression of target genes associated with migration and invasion, which were downregulated in the results of the transcriptome sequencing after SNHG5-overexpression.  < 0.05; (e, f) Western blot and immunofluorescence analysis evaluated the expression of EMT markers in control and A549 cancer cells after transfection with SNHG5 or siRNAs.  < 0.05.

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