LncRNA SNHG5 Suppresses Cell Migration and Invasion of Human Lung Adenocarcinoma via Regulation of Epithelial-Mesenchymal Transition

Figure 5

Overexpression of SNHG5 inhibits EMT, preventing LAD cells from migrating and invading. (a) After TGF-β1 or vehicle control treatment, qRT-PCR was used to examine the relative fold change of SNHG5 expression in control and SNHG5-overexpressing A549 cells.  < 0.001. (b) After TGF-β1 exposure, qRT–PCR was used to examine the relative fold change of EMT markers in normal and SNHG5-overexpressing A549 cancerous cells. (c) Western blot analysis was used to assess the level of expression of markers in control and SNHG5-overexpressing A549 cancer cells after TGF-β1 treatment. (d) The inverted microscope determined the morphological changes in control and SNHG5-overexpressing A549 cells after TGF-β1 or vehicle control treatment. (e, f) Western blot and qRT–PCR analysis of EMT-related transcription factors in normal and SNHG5-overexpressing A549 cancer cells following TGF-β1 or vehicle control treatment. (g–i) Transwell and wound-healing experiments were conducted on control and SNHG5-overexpressing A549 cancer tissues after TGF-β1 treatment. Wound-healing distance was measured and expressed as a percentage of the distance at zero hours. a:  < 0.01; b:  < 0.01; c:  < 0.001; d:  < 0.01; e:  < 0.001; f:  < 0.001.

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