Retinoblastoma-Binding Protein 5 Regulates H3K4 Methylation Modification to Inhibit the Proliferation of Melanoma Cells by Inactivating the Wnt/β-Catenin and Epithelial-Mesenchymal Transition Pathways

Figure 1

WSB2 was bound directly to RBBP5 and negatively regulated it. (a) STRING (“https://string-db.org”), an online database, showed that WSB2 was bound to the RBBP5 protein. (b, c) Western blotting was used to verify WSB2 protein levels after WSB2 knockdown. (b, d, e) Western blotting was used to explore the changes in RBBP5 and H3K4me3 protein after WSB2 knockdown. (f) Co-IP and western blotting were performed to evaluate the interaction between WSB2 and RBBP5. Data are represented as mean ± SD of three independent experiments. ; ns, no significance. (g, h) Immunohistochemical staining of RBBP5 and WSB2 in 104 cases of melanoma tumor tissues. Representative images are shown. Scale bar: 50 µm. The correlation between RBBP5 and WSB2 was calculated (i, j). n = 104, Pearson chi-square test.