The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses

Figure 3

The control purine metabolism-related gene PPAT can affect HCCC-9810. (a) qRT-PCR and Western blot were used to detect the expression of PPAT. (b) qRT-PCR and Western blot were used to measure the expression of IMPDH1. (c) Colony formation assay was performed to test the cloning ability of HCCC-9810. (d) Apoptosis rate of HCCC-9810 was analyzed by flow cytometry. (e) Transwell was used to detect migration of HCCC-9810. (f) HCCC-9810 invasion was detected by transwell. (g) Western blot was used to detect epithelial-mesenchymal transformation (EMT) markers of E-cadherin, N-cadherin, and vimentin. (h) The tumor stem cell markers CD133 and SOX2.  < 0.05 compared with the si-NC group. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test.

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