The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses

Table 3

Primer sequences.

Gene	Gene ID	Sequences (5′-3′)
H-PRPS1	5631	F: TGA​GCC​GCA​GTA​GTT​CTT​CAG
		R: GGC​AAC​AGT​TCT​TCA​ACT​GGC
H-PPAT	5471	F: TGG​TAG​CTG​GTA​GGG​TTG​GA
		R: AGG​CTA​GCC​AAT​GCT​TGT​GT
H-PAICS	10606	F: CAG​CAC​CCA​ACA​GTA​GCG​TA
		R: AGC​GAG​GCA​GAA​CTC​AAG​TC
H-PFAS	5198	F: ACG​ATT​CGA​GGT​GCT​CTG​TG
		R: CCT​TGC​AGT​TTC​CTC​CGA​GT
H-GAPT	202309	F: CAC​CCG​GTG​TCG​GTT​TCA​TT
		R: CCT​TTC​CAG​GCC​AGC​GTA​TGT​T
H-GMPS	8833	F: TGT​CAT​GAA​GCT​TCC​GTG​TT
		R: TCC​CCC​GTT​AAC​ATT​GAG​CA
H-ADSS	11566	F: AAG​GAG​CTA​CCT​GTC​AAC​GC
		R: CCA​ACG​CCA​GCT​GAA​AAC​TC
H-ADSL	158	F: TAA​ATT​CCG​GAC​ATG​GCG​GC
		R: CTG​CAG​CTT​TTG​GAC​AGC​AG
H-ATIC	471	F: CGG​CCA​GCT​CGC​CTT​ATT​TA
		R: CCA​AGA​GCG​GTC​AGG​TTT​CT
H-IMPDH1	3614	F: CGA​CAC​CCC​GTT​CAG​AAC​TAT
		R: TCA​GGT​AGT​CCG​CCA​TGC​TA
H-IMPDH2	3615	F: AGG​CGG​TAT​TGG​CTT​CAT​CC
		R: GCC​TGT​GTC​TGT​GAT​TGG​GA
M-PPAT	231327	F: GAA​GGA​GGC​AGT​CAG​TAG​CG
		R: AGT​CTC​CAG​AAG​CGA​TGC​AC
M-IMPDH1	23917	F: CTA​CGA​GCC​GGA​GAG​CAT​GG
		R: TGT​CAG​GTC​CAC​TTC​ATC​AGC
H-N-cadherin	35070	F: GGG​AAA​TGG​AAA​CTT​GAT​GGC​A
		R: TGG​AAA​GCT​TCT​CAC​GGC​AT
H-vimentin	7431	F: CCC​TTG​ACA​TTG​AGA​TTG​CCA​CC
		R: ACC​GTC​TTA​ATC​AGA​AGT​GTC​CT
H-E-cadherin	114424	F: ATT​TTT​CCC​TCG​ACA​CCC​GAT
		R: TCC​CAG​GCG​TAG​ACC​AAG​A
H-ACTINB	60	F: ACC​CTG​AAG​TAC​CCC​ATC​GAG
		R: AGC​ACA​GCC​TGG​ATA​GCA​AC
M-ACTINB	11461	F: ACA​TCC​GTA​AAG​ACC​TCT​ATG​CC
		R: TAC​TCC​TGC​TTG​CTG​ATC​CAC