Effect of Exosomal lncRNA MALAT1/miR-370-3p/STAT3 Positive Feedback Loop on PI3K/Akt Pathway Mediating Cisplatin Resistance in Cervical Cancer Cells

Figure 3

STAT3 induced the expression of MALAT1 in cisplatin-resistant CC cells. (a) Jaspar was used to predict the STAT3 binding site of the MALAT1 promoter region. (b) qRT-PCR was used to detect the level of STAT3 in patients with CC and adjacent cervical tissues. (c) The relative expression of STAT3 in cisplatin-sensitive group and cisplatin-resistant group in CC patients. (d) The expression of STAT3 in drug-resistant cells and parent cells was detected by qRT-PCR. (e) After low or high expression of STAT3, the expression of MALAT1 in CC cisplatin-resistant cells was detected by qRT-PCR. (f) Pearson’s analysis was used to analyze the correlation between STAT3 and MALAT1 in CC-resistant tissues. (g) STAT3 binding sites were analyzed by ChIP assay. (h) Schematic diagram of luciferase reporter plasmid constructed. (i) The relative luciferase activity was detected by dual-luciferase reporter assay. . All experiments were performed at least three times.

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