Effect of Exosomal lncRNA MALAT1/miR-370-3p/STAT3 Positive Feedback Loop on PI3K/Akt Pathway Mediating Cisplatin Resistance in Cervical Cancer Cells

Figure 4

miR-370-3p was the downstream miRNA of MALAT1 and was negatively regulated by MALAT1. (a) The database showed that miRNAs had conserved binding sites with MALAT1 and STAT3. (b) The expression of miR-370-3p after overexpression of MALAT1. (c) The expression of miR-370-3p in CC patients and paracancerous cervical tissues. (d) The relative expression of MALAT1 in cisplatin-sensitive group and cisplatin-resistant group in CC patients. (e) The relative expression of miR-370-3p in CC cell lines was detected by qRT-PCR. (f) There were conservative binding sites between MALAT1 and miR-370-3p. (g) Dual-luciferase reporter assay was used to detect the binding of MALAT1 to miR-370-3p. (h) RIP assay was used to detect the binding of MALAT1 and miR-370-3p. (i) The effect of MALAT1 on miR-370-3p was detected by qRT-PCR. Hela/R and C33A/R cells were co-transfected with MALAT1 low expression plasmid and miR-370-3p inhibitors. (j) qRT-PCR was used to detect the expression of MALAT1 and miR-370-3p. (k) CCK-8 assay was used to detect cell proliferation. (l) apoptosis was detected by flow cytometry. , , . All experiments were performed at least three times.

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