miR-4757-3p Inhibited the Migration and Invasion of Lung Cancer Cell via Targeting Wnt Signaling Pathway

Figure 3

miR-4757-3p regulated Wnt5a and Wnt8b expressions and activated the Wnt signaling pathway. (a) Predicting binding sequences of miR-4757-3p and Wnt5a. (b) Predicting binding sequences of miR-4757-3p and WNT8B. (c) The luciferase reporter assay demonstrated that Wnt5a or Wnt8b 3′ untranslated region-WT was targeted by miR-4757-3p. (d) Real-time PCR analysis of mRNA levels of Wnt5a and Wnt8b in A549 cell transfection with mimics. (e) Real-time PCR analysis of mRNA levels of Wnt5a and Wnt8b in A549 cell transfection with inhibitors. (f) Real-time PCR analysis of β-catenin expression in A549 cell transfection with mimics or inhibitors. .

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