GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis

Figure 2

Lentivirus-mediated GINS2 knockdown specifically inhibited GINS2 expression in OS cells. (a) Saos-2, HOS, and U2OS infected with GINS2 -shRNA or shCtrl lentivirus and nontransfected cells were examined by light microscopy and fluorescent microscopy 3 days after infection (100×). More than 80% of cells expressed GFP. (b–e) Efficient knockdown of GINS2 in OS cell lines. Saos-2 (b), U2OS (c), and HOS (d) cells were transduced with lentivirus expressing control or GINS2 shRNA. The knockdown efficiencies were detected with RT-qPCR and WB. All data were presented as the mean ± SD, statistical significance was analyzed by Student’s t-test and one-way ANOVA, , .

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