Ubiquitin-Specific Peptidase 8 Modulates Cell Proliferation and Induces Cell Cycle Arrest and Apoptosis in Breast Cancer by Stabilizing Estrogen Receptor Alpha

Figure 3

USP8 depletion or USP8 inhibitor inhibits BC cell proliferation and migration. (a) Inhibition of proliferation by DUB-IN-3 from 0 to 10 μM. IC50 = 4.452 μM. (b) Cell viability of MCF7 with 1 μM or 2 μM USP8 inhibitor. (c) Cell viability of MCF7 and T47D treated with 1 μM or 2 μM USP8 inhibitor in combination with 1 μM tamoxifen. (d, e) USP8 depletion increased the sensitivity of MCF7 and T47D to tamoxifen. Cells were treated with the indicated concentration of tamoxifen. (f, g) USP8 depletion inhibited the proliferation of BC cells. (h, i) USP8 depletion inhibited the migration of BC cells. (j, k) USP8 depletion decreased the clone-formation capability of BC cells. (l) Transwell migration and invasion assay of BC cells.

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