Ubiquitin-Specific Peptidase 8 Modulates Cell Proliferation and Induces Cell Cycle Arrest and Apoptosis in Breast Cancer by Stabilizing Estrogen Receptor Alpha

Figure 4

Induction of cell apoptosis and cell cycle arrest by USP8 knockdown and GSEA results of USP8. (a) Flow cytometry analysis of cell apoptosis in MCF7 and T47D treated with shUSP8 or shControl. PI PE-A stands for the fluorescence intensity of propidine iodide (PI), and FITC-A stands for the fluorescence intensity of fluorescein isothiocyanate (FITC)-labeled Annexin V. (b) Statistical analysis revealed the apoptotic rate (%) of both cell lines. (c) Flow cytometry analysis of the cell cycle in MCF7 and T47D treated with shUSP8 or shControl. Percentages (%) of cell populations at different stages of the cell cycle. (d) Statistical analysis of the percentages (%) of cell populations at different stages of the cell cycle in MCF7 and T47D treated with shUSP8 or shControl. (e) Immunoblot assay of apoptosis-related and cell cycle-related proteins in MCF7 and T47D treated with shUSP8 or shControl. (f) Enriched gene sets in the c2 KEGG gene collection in high-USP8-expression samples according to GSEA. Each line = a gene set; upregulated genes are located on the left, near the origin of the coordinates; downregulated genes are located on the right of the x-axis. Only gene sets with NOM value <0.05 and FDR q-value <0.25 were considered significant. Only top gene sets are shown in the plot. (g) Enriched gene sets in the c2 KEGG gene collection in low-USP8-expression samples according to GSEA. (h) Enriched gene sets in the hallmark collection in high-USP8-expression samples according to GSEA. (i) Enriched gene sets in the hallmark collection in low-USP8-expression samples according to GSEA. , ; , ; , .

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