Altered Hepatic Lipid Metabolism Contributes to Nonalcoholic Fatty Liver Disease in Leptin-Deficient Ob/Ob Mice

Table 1

qRT-PCR primer sequences.

Gene	Forward sequence	Reverse sequence
ACC	AACATCCCCACGCTAAACAG	CTGACAAGGTGGCGTGAAG
CPT-1	AGTGGCCTCACAGACTCCAG	GCCATGTTGTACAGCTTCC
Cyclophilin	ATGTGGTTTTCGGCAAAGTT	TGACATCCTTCAGTGGCTTG
FAS	CCCTTGATGAAGAGGGATCA	GAACAAGGCGTTAGGGTTGA
IL-1β	TCACAGCAGCACATCAACAA	TGTCCTCATCCTGGAAGGTC
IL-6	ACCAGAGGAAATTTTCAATAGGC	TGATGCACTTGCAGAAAACA
PGC-1α	ATGTGTCGCCTTCTTGCTCT	ATCTACTGCCTGGGGACCTT
PPAR-α	CAGTGGGGAGAGAGGACAGA	AGTTCGGGAACAAGACGTTG
PPAR-γ	GATGGAAGACCACTCGCATT	AACCATTGGGTCAGCTCTTG
SCD-1	GCTGGGCAGGAACTAGTGAG	GAAGGCATGGAAGGTTCAAA
SREBP-1c	ATCTCCTAGAGCGAGCGTTG	TATTTAGCAACTGCAGATATCCAAG
TFAM	TCCAAGCCTCATTTACAAGC	CCAAAAAGACCTCGTTCAGC
TGF-β	AAGTTGGCATGGTAGCCCTT	GCCCTGGATACCAACTATTGC
TNF-α	ATGGGCTTTCCGAATTCAC	GAGGCAACCTGACCACTCTC