Optimization of Storage Temperature for Cultured ARPE-19 Cells

Figure 4

Cultured RPE cells were stored for seven days at 4°C, 8°C, 12°C, 16°C, 20°C, 24°C, 28°C, 32°C, and 37°C, and viability was assessed with a calcein-acetoxymethyl ester reagent. The bar chart shows the percentage of viable cells after storage compared to control cells (100%). *𝑃<0.01 compared to 4°C, 8°C, 28°C, and 37°C; 𝑃<0.05 compared to 24°C and 32°C. ^†𝑃<0.01 compared to 4°C, 8°C, and 24°C–37°C; 𝑃<0.05 compared to 12°C. ^‡𝑃<0.01 compared to 4°C, 8°C, and 24°C–37°C. Error bars represent the standard deviation of mean values.