Optimization of Storage Temperature for Cultured ARPE-19 Cells

Table 1

Characterization of retinal pigment epithelial cells.

Markers	Specificity	Investigator criteria for positive staining	Investigator A and B agreement
			Significance level of correlation (r) between investigators	95% CI of difference between investigators
RPE65	Differentiated cells (cytosol/membrane)	Stained cytosol	𝑃<0.001	−0.1% to +0.0%
PCNA	Proliferating cells (nucleus)	Stained nucleus	𝑃<0.001	−3.3% to +0.8%
Caspase-3	Apoptotic cells (mainly cytosol)	Stained cytosol	𝑃=0.044	−0.2% to +0.0%

CI: confidence interval; r: Pearson’s correlation coefficient.