Intravitreal and Subconjunctival Melphalan for Retinoblastoma in Transgenic Mice
Figure 5
Hypoxia and vasculature reductions after treatment with 1 μg intravitreal injections after 1 week and 3 weeks and following subconjunctival serial injections with 10 μg 2x wk for 3 weeks or a 1-time administration of 100 mcg. Hypoxia showed a statistically significant reduction after treatment with 1 μg intravitreal melphalan at 1 week () and at 3 weeks following a 100 μg maximum dose subconjunctival treatment (). Blue: DAPI stain for all the cell nuclei; green: pimonidazole stain for hypoxic regions. Red (extrAvidin-cy3) stains mature vasculature and green (anti-endoglin) stains neovessels. Pictures were obtained at magnification ×200 high power field.
