Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis

Figure 5

Effect of apelin on cell viability and migration under normoxia. (A) The Edu proliferation assay. Apollo staining (red) represents proliferating cells, and DAPI (blue) staining nuclei. Compared with the control group, the number of proliferating cells treated with apelin (10 or 100 ng/mL) increased significantly. Scale bar = 200 µm. (B) The folds of apelin-treated cell viability compared with the control group (, versus untreated control); (C) and (D) pericyte migration in response to apelin treatment was measured using the transwell assay (a: control; b: 1 ng/mL; c: 10 ng/mL; d: 100 ng/mL, versus untreated control). The data are expressed as means ± standard deviation (SD). Scale bar = 200 µm.