Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering

Figure 9

Human limbal epithelial cells on the DCL. Epithelial cells formed a stratified multilayer on the DCL. The cells visualized with DAPI (a) and merged with the bright field image (b). RT-PCR data showed that stratified epithelium cultured on DCL expressed progenitor and corneal epithelial cell markers (c). Scale bar indicates 50 μm.

(a)

(b)

(c)