Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats

Figure 1

The expression of proteins containing D-aspartic acid in the retinas of rats with ocular hypertension and normotension. (a) Total lysates of retinas of rats with ocular hypertension (OH) and with normotension (NT) were applied to 10% acrylamide gel (SDS-PAGE) and blotted with anti-D-aspartic acid antibody. Blotting with anti-actin antibody was performed to standardize the total volume of applied proteins. The expression volumes of proteins containing D-aspartic acid were corrected with each actin expression, and the ratios at OH and NT are shown in the lowest panel. The black arrow, labelled D-Asp, shows a band with stronger expression in OH than NT. (b) Total lysates of retinas of OH and NT were blotted with anti-D-aspartic acid antibody (a) and stained with Ponceau solution (b).

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