Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy

Figure 1

Quantification of the vascular area of retina at 4 dpf using Fiji-ImageJ software. An example of a retinal vascular image: use Fiji-ImageJ “area measurement” function under “Analyze” to measure the relative vascular vessel area over the retinal area. (a) Total retinal area by drawing a uniform circle of 120 pixel diameter = 11304 pixel area. (b) Draw and measure areas outside the vascular vessels = 2393 + 1256 + 638 + 1640 + 259 + 366 = 6552. Then, calculate % vascular vessel area of the retina = (11304 − 6552)/11304 × 100% = 42.0%. Branch point of retinal vessels.

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(b)