Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activator Using Human-Induced Pluripotent Stem Cells

Figure 3

Effects of 24-hour rtPA exposure on cell morphology. ZO-1-stained (green) and DAPI-stained (blue) confocal images ((A) 0 μg/ml, (B) 20 μg/ml, and (C) 100 μg/ml) and scanning electron microscopy images ((D) 0 μg/ml and (E) 100 μg/ml) of hfRPE (a), hiPSC-RPE ((b) 454E2), and hiPSC-RPE ((c) 253G1) treated with 0, 20, and 100 μg/ml rtPA. Scale bar, 10 mm. (d) ZO-1-stained (green) and DAPI-stained (blue) stained confocal images ((A) dissociated cells and (B) aggregated cell) and scanning electron microscopy images ((C) basement membrane-like matrix, (D) aggregated cells, and (E) Transwell® insert membrane) of hiPSC-RPE (454E2) treated with 2000 μg/ml rtPA. Scale bar, 10 mm.

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