The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane

Table 2

Comparison of the mean thicknesses of the Descemet’s membrane obtained by digital microscopy in the different regions of normal corneas and corneas from failed grafts due to rejection.

Mean thickness of the Descemet’s membrane (μm, 40x)
	Central	Nasal	Temporal	value
Normal corneas (mean ± SD)	8.410^a (±2.016)	14.363^b (±4.885)	13.337^b (±3.484)	<0.001
Corneas from failed grafts due to rejection (mean ± SD)	8.545^a (±2.712)	9.883^a (±3.186)	9.506^a (±2.625)	0.101

n = number of cases; SD = standard deviation; μm = micrometer; 40x = microscope magnification; ^a,b = letters to identify the differences between the groups in the central, nasal, and temporal measurements of normal corneas and corneas from failed grafts due to rejection. The Kolmogorov–Smirnov test was used to test the equality of probability distribution, and ANOVA and Tukey’s test were used for comparisons between multiple variables of the samples.