In Vitro Screening and Transfection Concentration Optimization of Cynomolgus Monkey IκBα-siRNA

Figure 4

Western blotting for detection of IκBα protein expression after NC-siRNA and three pairs of IκBα-siRNA were transfected into CM cells and TM cells, respectively, at 10 nM. (a) The relative protein expression of IκBα of No. 1, No. 2, and No. 3 groups were significantly lower compared to that of the NC-siRNA group in CM cells. The three pairs of IκBα-siRNA could all inhibit the IκBα protein expression, and the inhibitory rate of No. 2 was higher than that of the other two pairs. (b) The relative protein expression of IκBα of No. 1, No. 2, and No. 3 group was significantly lower compared to that of the NC-siRNA group in TM cells. The three pairs of IκBα-siRNA could all inhibit the IκBα protein expression, and the inhibitory rate of No. 2 was higher than that of the other two pairs.

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