|
| Anatomical | In vivo CLSM | Ex vivo CLSM | H&E staining |
|
| Stratum corneum ↓ 0–10 μm | Highly reflective, polygonal, flat, nonnucleated cells (25 μm-35 μm) | Medium reflection, polygonal, flat, nonnucleated cells | Multiple layers of pink, flat, nonnucleated keratinocytes |
| Stratum granulosum ↓ 10–20 μm | 1–3 layers, flat, low-reflective cytoplasm (20–30 μm) | 1–3 layers, flat, medium-reflective cytoplasm, highly reflective nuclei | Multiple layers of pink cytoplasm with dark granules and dark blue or purple nuclei |
| Stratum spinosum ↓ 20–100 μm | 2–7 layers, polygon, high-reflective cell junctions, and low-reflective cells with invisible nuclear (15–25 μm) | 2–7 layers, polygon, high reflective nucleus, low-reflective cytoplasmic cells | Multilayer, purple nuclei in the center of the pink cytoplasm |
| Stratum basale ↓ 50–100 μm | Monolayer, cube-shaped, highly reflective nuclei and medium-reflective cytoplasmic cells (10 μm) | Same as in vivo CLSM | Monolayer, purple nuclei in the center of the pink cytoplasm, darker than stratum spinosum |
| Papillary dermis ↓ 60–120 μm | The low-reflection area is surrounded by a highly reflective layer, and highly reflective cells (inflammatory cells, red blood cell) are visible inside | Same as in vivo CLSM | Crispy pink collagen fibers surrounded by stratum basale |
| Reticular dermis | Low reflection background and high reflection net-like collagen fibers | Crispy medium reflective collagen fibers | Crispy pink collagen fibers |
| Hair follicle | Highly reflective hair shaft surrounded by highly reflective root sheaths with cells arranged in columns | Same as in vivo CLSM | Hair shafts as columns of pink to brownish keratinized cells |
| Glands of Zeis | Tubular high reflection duct and blurred medium reflection acini | Round, sharply demarcated structures filled with low-reflective cytoplasm, highly reflective nuclei | Opens into the acetabulum, large cells packed with lipids and centrally located nuclei |
| Glands of Moll | Unable to observe | 1–2 layers of small highly reflective nuclei cells, lining the low-reflective lumen | Located near the lash follicles within the margins of the lids, tubular composed of flat cells, others with cuboidal cells |
|
