The Effect of A2E on the Uptake and Release of Calcium in the Lysosomes and Mitochondria of Human RPE Cells Exposed to Blue Light

Figure 1

Human RPE cells and immunohistochemistry. (a) After being cultured for 3 days, primary human RPE cells were spindle-shaped or polygonal and the cytoplasm was filled with pigment particles. (b) Seven days later, the cells were spindle-shaped or irregular, with round and binuclear nuclei. (c) The third generation of human RPE cells had fewer pigment particles in the cytoplasm and presented as spindles or irregular polygons. (d) Primary mouse anti-human RPE65 antibodies and secondary goat anti-mouse IgG antibodies were used to immunostain third-generation RPE cells. Immunofluorescence staining was positive for RPE65.

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