Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia

Figure 1

Schematic representation of two CoCl₂ treatments. (1) Exposure of MIO-M1 cells to 100 µM CoCl₂ for 48 h followed by 24 h of recovery in a complete medium (DMEM + 10% FBS + 1% L-Glut + 1% Pen/Strep). (2) 24 h exposure to 100 µM CoCl₂ followed by 24 h of recovery. CTR: control sample. White arrow: medium added with CoCl₂. Black arrow: medium without CoCl₂. Black vertical lines indicate timepoints of cell collection.