Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway

Figure 4

CKIP-1 inhibits high glucose-induced autophagy. (a) The LC3 and Beclin-1 expression was estimated by Western blots. (b) The protein expression of p62. (c) Immunofluorescence of LC3-II in ARPE-19 cells with different treatments.  < 0.001 vs. NG.  < 0.05 and  < 0.001 vs. HG.  < 0.05 and  < 0.001 vs. HG + NC.  < 0.05 and  < 0.001 vs. HG + CKIP-1. NG, normal glucose (5.5 mM); MG, mannitol (20 mM); HG, high glucose (25 mM); NC, pcDNA3.1 vector negative control; CKIP-1, pcDNA3.1-CKIP-1; ML385 (5 μM), the Nrf2 inhibitor.

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