Circ-ADAM9 Promotes High Glucose-Induced Retinal Pigment Epithelial Cell Injury in DR via Regulating miR-338-3p/CARM1 Axis

Figure 2

Effects of si-circ-ADAM9 on HG-induced ARPE-19 cell injury. ARPE-19 cells were transfected with si-NC or si-circ-ADAM9 and then treated with HG. The cells treated with NG were used as control. (a) The expression of circ-ADAM9 was measured by qRT-PCR. (b) ELISA was utilized to detect the concentrations of IL-6 and TNF-α. MTT assay (c), EdU assay (d), and flow cytometry (e) were determined to measure cell proliferation and apoptosis. (f) The protein expression of Bax and cleaved-caspase-3 was detected by the WB analysis. (g, h) MDA level and SOD activity were examined to assess cell oxidative stress. , , and .

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