Complement 3a Mediates CCN2/CTGF in Human Retinal Pigment Epithelial Cells

Figure 2

Effect of C3a on mRNA and protein levels of CCN2/CTGF in cultured RPE cells. Total RNA was extracted, and CCN2/CTGF mRNA was evaluated by RT-PCR analysis. The ratio of the abundance of the target mRNA to GAPDH was evaluated by densitometric analysis (a). , versus negative control cells; , versus cells with 0.3 μM C3a for 72-hour time. ELISA analysis to assess the effect of C3a on protein levels of CCN2/CTGF in cultured RPE cells (b). The expression level was normalised to the negative control. , versus negative control; , versus cells with 0.3 μM C3a for 72-hour time. The data are presented as the mean ± SD (n = 4).

(a)

(b)