miR-486-5p Restrains Extracellular Matrix Production and Oxidative Damage in Human Trabecular Meshwork Cells by Targeting TGF-β/SMAD2 Pathway

Figure 1

miR-486-5p was downregulated in H₂O₂-stimulated TMCs. (a) After TMCs were treated for 12 h with varying concentrations of H₂O₂ (0–400 μM), viability was detected by MTT. After TMCs were treated with 300 μM H₂O₂ for 0–24 h (b) viability was detected by MTT. (c) The miR-486-5p level was assessed by RT-qPCR. Transfected and nontransfected cells were stimulated with 300 μM H₂O₂ for 12 h and nontreated cells acted as the control. (d, e) Overexpression efficiency of miR-486-5p mimics in nontreated cells and H₂O₂-treated TMCs was evaluated by RT-qPCR. (f) Viability was detected by MTT. ; ; .

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