miR-486-5p Restrains Extracellular Matrix Production and Oxidative Damage in Human Trabecular Meshwork Cells by Targeting TGF-β/SMAD2 Pathway

Figure 2

miR-486-5p overexpression mitigated H₂O₂-induced injury in TMCs. After TMCs were disposed with 300 μM H₂O₂ alone, 300 μM H₂O₂ plus NC mimics alone, or 300 μM H₂O₂ plus miR-486-5p mimics, (a) apoptosis was assessed by flow cytometry; (b) protein expression of cleaved caspase-3/9 was determined by western blotting; (c, d) the SOD and MDA levels were assayed; (e, f) content of collagen I, fibronectin, and laminin was tested by the immunofluorescence staining assay; and (g) expression of collagen I, fibronectin, and laminin was tested by western blotting. ; .

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