miR-486-5p Restrains Extracellular Matrix Production and Oxidative Damage in Human Trabecular Meshwork Cells by Targeting TGF-β/SMAD2 Pathway

Figure 4

SMAD2 downregulation attenuated H₂O₂-induced injury in TMCs. After TMCs were disposed with 300 μM H₂O₂ alone, 300 μM H₂O₂ plus sh-NC alone, or 300 μM H₂O₂ plus sh-SMAD2, (a) the SMAD2 level was determined by RT-qPCR; (b) apoptosis was assessed by flow cytometry; (c) protein expression of cleaved caspase-3/9 was determined by western blotting; (d, e) the SOD and MDA levels were assayed; (f) expression of collagen I, fibronectin, and laminin was tested by western blotting. ; .

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