Application of High-Throughput Sequencing Technology in Identifying the Pathogens in Endophthalmitis

Table 2

Comparison of culture with HTS sequencing results.

Sequencing platform	Positive rate of sequencing	Positive rate of culture	The most abundant genus of sequencing	Dominant bacteria of culture	References
IlluminaHiSeq2500	60/75	18/75	Streptococcus, Staphylococcus	Streptococcus, Staphylococcus	[31]
IlluminaHiSeq2500	30/34	15/34	Streptococcus	Streptococcus	[32]
Applied Biosystems	30/88	20/88	Staphylococcus epidermidis	Staphylococcus	[33]
Illumina HiSeq 300	5/5	0/5	Pseudomonas, Staphylococcus	—	[34]
Illumina MiSeq	12/14	12/14	Staphylococcus epidermidis	Staphylococcus epidermidis	[35]
			Enterococcus faecalis	Enterococcus faecalis
SMART	42/46	24/50	Staphylococcus	Coagulase-negative Staphylococcus	[36]
Applied Biosystems	3/3	3/3	Case 1 Klebsiella pneumoniae	Case 1 Klebsiella pneumonia	[37]
			Case 2 Streptococcus galactiae	Case 2 Streptococcus alactiae
			Case 3 Candida parapsilosis	Case 3 Candida parapsilosis

SMART: single molecule real-time sequencing.