Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential

Figure 1

Drug susceptibility assays against C. albicans in the presence of Ses. (a) Spot assay of C. albicans reference strains (ATCC 10261, ATCC 24433) and clinical isolates (D1, D2, D4, D7, D18, and D20) in the absence (control) and presence of Ses. (b) Disc diffusion assay against C. albicans reference strains (ATCC 10261, ATCC 24433) and clinical isolates (D1, D2, D4, D7, D18, and D20) of C. albicans and their respective zone of inhibitions in the absence (control) and presence of Ses. For control, discs were spotted with the solvent of Ses described in Section 2. (c) Broth microdilution assay to determine the MIC₈₀ of C. albicans reference strains (ATCC 10261, ATCC 24433) and clinical isolates (D1, D2, D4, D7, D18, and D20) in presence of Ses. Data was quantitatively displayed with colour (see colour bar), where each shade of colour represents relative optical densities of the cell. The minimum drug concentration that inhibits growth by 80% relative to the drug-free growth control is indicated as MIC₈₀ for each strain.