Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential

Figure 4

Drug susceptibility assay against C. albicans and non-albicans speciesat alkaline pH. (a) Spot assay of C. albicans, C. tropicalis, and C. glabrata at pH 10 and C. krusei and C. parapsilosis at pH 8 in the absence (control) and presence of Ses. (b) Disc diffusion assay against C. albicans, C. tropicalis, and C. glabrata at pH 10 and C. krusei and C. parapsilosis at pH 8 and their respective zone of inhibitions in the presence of Ses. For control discs were spotted with the solvent of Ses described in Section 2. (c) Broth microdilution assay to determine the MIC₈₀ at alkaline pH of C. albicans, C. tropicalis, and C. glabrata at pH 10 and C. krusei and C. parapsilosis at pH 8 in the presence of Ses. Data was quantitatively displayed with colour (see colour bar), where each shade of colour represents relative optical densities of the cell. The minimum drug concentration that inhibits growth by 80% relative to the drug-free growth control is indicated as MIC₈₀ for each strain.