Antimalarial Activity of Nigella sativa L. Seed Extracts and Selection of Resistance in Plasmodium berghei ANKA in a Mouse Model

Table 1

Summary of PCR programme.

PCR amplifying summaries	Temperature (°C)/time (min)
	PbCRT	PbMDR1
Initial denaturation	95°C, 5 min	95°C, 5 min
Denaturation	95°C, 1 min	95°C, 1 min
Annealing temperature	49°C, 45 sec	48°C, 45 sec
Elongation	68°C, 6 min	68°C, 5 min
Final elongation	72°C, 10 min	72°C, 10 min
Primer (forward and reverse)	0.25 pmol/μl each	0.25 pmol/μl each
Mgcl2 (mM)	2.0	1.5
dNTPs (mM)	0.2	0.2
Cycles	35	35
Infinite hold	4°C	4°C