Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci)

Figure 3

Effect of auranofin on the disulfide reductase activities of mitochondrial TGR. An enzyme aliquot (about 25 ng) was preincubated at ${25}^{°}\text{C}$ in the presence of 100 $\mu$M NADPH and inhibitor as described under Materials and Methods. To start the reaction either GSSG or DTNB was added. Final concentrations of the disulfide substrate were 40 $\mu$M GSSG (); and 1 mM DTNB ().