Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci)

Figure 6

Antioxidant and protective activities of mTGR. (a) H₂O₂ reductase activity. The NADPH-dependent reduction of H₂O₂ by mTGR was assayed at ${25}^{°}\text{C}$ in the presence of 100 $\mu$M NADPH. (b) Protection of glutamine synthetase. A mTGR aliquot (10 nM) was incubated along E. coli glutamine synthetase (150 nM) in the presence of a mixed-function oxidation system in a final volume of 50 $\mu$L. After 10 minutes, 2 mL of the $\gamma$-glutamyltransferase assay mixture was added. Additional details are described under Materials and Methods. (a) positive control; (b) negative control; (c) Full mixture without mTGR; and (d) Full mixture.

(a)

(b)