Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci)

Table 1

Summary of the mTGR purification procedure.

Fraction	${\text{Vol}}_{\text{Total}}$ (ml)	Protein (mg)	Specific Activity (mU*/mg)			Activity ratio	Activity (mU)	Purication (fold)	Yield (%)
			GSSG	DTNB	HED	DTNB/GSSG		DTNB
Crude extract	13.0	33.5	11.7	35,0	128.5	3.0	1172.5	1.0	100
DEAE-Sephacel	3.3	9.37	34.0	75.2	138.0	22	704.6	2.1	60
HA-Ultrogel	3.2	2.23	38.7	184.0	532.1	4.7	410.3	5.3	35
Cibacron-Blue	1.72	0.186	229.0	1091.0	2242.8	4.7	202.9	31.2	17

An enzyme mU is defined as the amount of protein needed to oxidize one nmol of NADPH per minute at ${25}^{°}$C.
Date obtained at ${25}^{°}$C and pH 7.8.