Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo
Figure 4
Treatment of mice with L-NIL, an iNOS-specific inhibitor, had no effect on parasitemia but a moderate effect on the production of NO in spleen cell cultures. Groups of 6 C57BL/6 mice were treated i.v. with L-NIL (20 or 40 mg/ body weight) on day −1, 1, 2, and 3 postinfection. Mice were infected i.p. with T. congolense TC13 on day 0. Parasitemia (a) was determined. On day 6 postinfection, spleen cells were cultured at an optimal cell density (/mL) for 48 hours. Nitrite (b) in culture supernatants was measured by the Griess reaction. **; ***.
