Improved Method for the Detection and Quantification of Naegleria fowleri in Water and Sediment Using Immunomagnetic Separation and Real-Time PCR

Table 5

Estimated concentration of N. fowleri in water and sediment samples.

Sample ID	No. of amebas seeded	Average CT value	Estimated concentration (amebas/L)
GA water	14	39.1	8
GA water	63	38.5	15
GA water	388	35.1	183
GA water	4,900	31.9	1,224
GA water	11,300	29.8	5,902
GA water	129,000	27.9	18,620
FL5 water	NA	40.6	2.7
FL6 water	NA	38.2	15
MN9 sediment	NA	38.3	13
WB saline control	9,800	28.8	7,900
WB saline control	37,000	27.8	15,000