Partial Purification of Integral Membrane Antigenic Proteins from Trypanosoma evansi That Display Immunological Cross-Reactivity with Trypanosoma vivax
Figure 2
The 64 kDa band that is enriched in the fraction corresponds to the previously reported p64 antigen [10, 11], that is partially responsible for the cross-reaction between T. evansi and T. vivax. The p64 antigen was observed in the parasite homogenate (H), soluble (S), and Triton X-100 solubilized () fractions. No p64 was obtained in the resulting pellet after treatment with Triton X-100 (). (a) Western blot using polyclonal anti-p64 antibodies prepared in rabbit sera. (b) Western blot using polyclonal anti-p64 antibodies prepared in mice ascitic fluid. M = protein markers.
