Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex

Table 2

Primer sequences used for semiquantitative and real-time PCR.

Gene	Accession number	Primers (5′-3′)	Size of PCR product (bp)
tps	/KJ560557	For. GAAGTTACGTCAATCAATATGAGAAGG/ACTACGCATCACAAGCAACG	163
		Rev. TTCAGGTCCACCCACCCATC/CGCTTGACGTATCAATGGAA
tpp	/KJ560558	For. GTTTTCGCTTGTCTGCTCACG/TACGAAAGCATTCCAACGTG	193
		Rev. TCGTTAGCGGCATTTCCTG/ATCGGATGATACGCTGCAAG
gs	JX173686	For. GCCACCGATTTGCACACACAA	173
		Rev. CAGCCACGCAAATTCCTCA
gp	JX941465	For. GGATTCGCACACAACAATACTA	193
		Rev. RCTGCGTCTTCTCGATCTCTTG
18S	U81575	For. ACCAGTAACGAAAGCGTGTG	211
		Rev. GCTGTTGGAAGGAAGAACGA

This sequence data was used to design primers for real-time PCR.