Identification, Characterization, and Structure of Tm16 from Trichuris muris

Figure 1

Identification of Tm16 by 2D gel electrophoresis on 8–16% Tris-HCl criterion gel. The first dimension was isoelectric focusing while the second dimension was SDS-PAGE. Ten independent protein spots were excised and sent for identification. Tm16 identified in spots 7 and 9. (a) Gel was stained with Coomassie brilliant blue. (b) Western blot of corresponding gel primary antibody was mouse ES immune sera and secondary antibody was anti-mouse IgG HRP.

(a)

(b)