Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Diagnosis of Schistosoma mansoni Infection in Faecal Samples

Figure 1

(a) Lanes 2 and 3 represent adult worm DNA, lane 4 represents miracidia DNA, lane 5 represents schistosome egg DNA, lanes 6 and 7 represent cercarial DNA, lane 8 represents a –ve control (containing a premix only), and lanes 1 and 9 represent the molecular marker. (b) LAMP amplification reactions in a tube with products stained with SYBR Green I stain, and samples visualized directly with unaided eye. Tubes 1-7 in Figure 1(b) correspond to lanes 2-8, respectively, in Figure 1(a).

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