Angiotensin II Induces Differentiation of Human Neuroblastoma Cells by Increasing MAP2 and ROS Levels

Figure 2

Effect of Ang II-treated neuroblastoma cells on MAP2 and NF-H expression levels. Cells were incubated with Ang II (600 nM) for 24 h, and the levels of MAP2 and NF-H proteins were measured by flow cytometry using specific monoclonal antibodies. (a) MAP2 levels increased significantly when the cells were incubated with Ang II compared to untreated. (b) No significant differences were observed in the levels of NF-H when the cells were incubated with Ang II compared to untreated. Each bar represents the average of four experiments. Vertical lines at each bar represent SEM (). MFI: mean fluorescence intensity.