Angiotensin Receptor Blocker and Neprilysin Inhibitor Suppresses Cardiac Dysfunction by Accelerating Myocardial Angiogenesis in Apolipoprotein E-Knockout Mice Fed a High-Fat Diet

Figure 6

Myocardial angiogenesis. (a) Representative microphotographs of the left ventricle, with immunohistochemical staining for CD34, and a quantitative analysis for the percentage of the CD34-positive cell area in each group. mRNA expression levels of (b) CD34, (c) VEGFA, (d) MCP1, (e) ATP2a2, (f) VCAM-1, (g) β catenin, and (h) VE-cadherin in each group. CD34: clusters of differentiation 34; VEGFA: vascular endothelial growth factor A; MCP1: monocyte chemotactic protein 1; ATP2a2: gene name of sarcoplasmic/endoplasmic reticulum calcium adenosine triphosphatase 2; VCAM-1: vascular cell adhesion protein 1; VE-cadherin: vascular endothelial-cadherin; CTL: control group; VAL: valsartan group; SAC: sacubitril group; VAL/SAC: valsartan plus sacubitril group. CTL (), VAL (), SAC (), and VAL/SAC () were investigated. , †, and ‡ indicate significant differences compared with CTL, VAL, and SAC, respectively.

(a) CD34 staining

(b) CD34

(c) VEGFA

(d) MCP1

(e) ATP2a2

(f) VCAM-1

(g) β catenin

(h) VE-cadherin